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        -----Original Message-----
        From:   Patty Tam [SMTP:[log in to unmask]]
        Sent:   Wednesday, September 16, 1998 12:51 AM
        To:     [log in to unmask]
        Subject:        FITC/Tx Red Labelled protein stuck on cibacron blue
column!

        Hello!  I need some help.  I labelled my protein with texas red and
FITC,
        (two separate batches of protein, singly labelled).  I put the labelled
        protein on an Affi-Gel Blue(Cibacron Blue) column and I managed to get
        some of the labelled protein off the column using 0.6M NaCl, but the
        majority is still stuck on the column.
        I think the label(Texas red or FITC) is interacting with the Cibacron
        Blue.  I tried eluting with 1.5M ammonium acetate, but that didn't work
        either.

        Does anyone have any suggestions on how to get my protein off the
        column, or know of any other newsgroups that could be of help?

        Thank you!

        Patty Tam
        Graduate Student
        University of Toronto