> Basically, I have a post-grad student who would like to assess the
> architecture of bone (compact and spongy).  She is looking for a fluorescent
> dye that will enable imaging.  She doesn't need to disctiminate between the
> different cell populations but rather is looking at the arrangement of
> trabeculae, etc.  We have a krypton/argon laser with 488nm, 568nm and 647nm
> excitation.  We have a number of filters available for detecting emmision on
> our Leica TCS4d CLSM.
>
> Can anyone help me out with a dye please?

Hi Felicity,

Apparently, xylene orange is the stain you need (avail. BDH, Fluka,
Sigma, Kodak). Appears to be the same as xylenol orange... Sigma in
Australia have in stock if you can't find a dusty bottle in the histo lab.

BTW, can anyone recommend books / references on stains, that are BETTER than
Staining Procedures by G. Clark.  Would that Molecular Probes covered
all the older more traditional and non-fluorescent stains too.........

Ian.

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Dr Ian S. Harper
Confocal Microscopy Facility
Department of Biological Sciences
Monash University, Clayton, Vic 3168, Australia
Email: [log in to unmask]
Tel: +61 3 9905 5635;  Fax: +61 3 9905 5613
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