CONFOCALMICROSCOPY Archives

September 1998

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Date:
Fri, 4 Sep 1998 09:41:52 +0200
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Confocal Microscopy List <[log in to unmask]>
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From:
Kees Jalink <[log in to unmask]>
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The Netherlands Cancer Institute, Amsterdam
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Confocal Microscopy List <[log in to unmask]>
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I've used Indocyanine green for patchclamp experiments (insect and
mammalian cells) and it works well. The words of Bill Mason should be
taken seriously: most microscopes not only have coatings that don't
transmit in the far red, but some also contain extra (undocumented) IR
filters in both fluorescent lamp attachment and CCD port. Just take out
the entire fluorescence attachment and stick it in a spectrophotometer.

regards, Kees

--

Kees Jalink Ph.D.
The Netherlands Cancer Institute, dept. of Cell Biology H1
Plesmanlaan 121 1066CX Amsterdam, the Netherlands
020-5121933 (tel)   / 020-5121944 (fax)
[log in to unmask] (email) / 0297-320248 (tel at home)

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