CONFOCALMICROSCOPY Archives

April 1999

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Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 27 Apr 1999 13:31:05 -0400
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Confocal Microscopy List <[log in to unmask]>
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Advanced Imaging Methodology Consultation
From:
Lutz Schaefer <[log in to unmask]>
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Hans van der Voort wrote:
>
> (message from a commercial vendor who couldn't resist this one, ;-))
>
Really...?
Well, you are right, the convolution with the pinhole is in most cases
circular symmetric thus simplifying it to a 1D convolution with some
pre-calculations and the three diffraction integrals are numerically
easy to manage as well. I suppose the question by the previous poster
was not meant to aquire a deconvolution system in order to calculate
optical sectioning width.

Anyhow, to what image volume size do the 2.1 seconds refer?

Best Regards
Lutz

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