CONFOCALMICROSCOPY Archives

April 1999

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Joachim Walter <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Sat, 24 Apr 1999 00:15:02 +0200
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On the LSM 410 you can create a color table in the pulldown menu
Image -> color tables -> user color table
You can create gradients in red green and blue, but you cannot freely
edit
the colors. However the color table is stored in a simple format: The
RGB
values for each intensity value (0...255) are stored as bytes. First
come
all values for the red channel from 0 to 255 then all green and all red.
You can create an arbitrary color table with a hex-editor, if you have
the
patience to do that:-)

Joachim



Khaled wrote:
>
> I was wondering if someone knew how to create pseudocolor on LSM410
> and 510. I am doing Ca imaging and would like to pseudocolor the
> images based on the level of fluorescence of the Ca sensitive dye.
> Thanks for your input.
>
> *********************************
> Khaled Machaca, Ph.D.
> Department of Cell Biology
> Emory University
> 1648 Pierce Drive
> Atlanta, GA 30322
> Voice: (404) 727-6260
> FAX: (404) 727-6256
> email: [log in to unmask]
> *********************************

--
------------------------------------------------------------------
  Joachim Walter
  Institut für Anthropologie und Humangenetik der LMU München
  AG Cremer
  Richard-Wagner-Straße 10/I
  D-80333 München               Tel. +49 - 89-5203-383
  Germany                       Fax. +49 - 89-5203-389

  [log in to unmask]
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