Dear all,

What extras do you need on a confocal (or any fluorescence microscope) to
do FRET?  I'm assuming you need:

 - laser/filter that gives correct excitation, e.g. below about 460nm for
CFP-YFP FRET
 - interference and narrow-band longpass filters appropriate to the
fluorochromes
 - software to do ratiometric imaging - esp. for something like CFP-YFP
where there is considerable overlap in emission from the two proteins

Is that it?

Reference to an idiot's guide to FRET would be appreciated.  Have checked
out latest articles in TiCB and TiPS, which focus on applications rather
than how to set it up.

Thanks all,


Rosemary White
Department of Biological Sciences
Monash University, Melbourne, Victoria 3168, Australia
phone  61-3-9905 5670    fax    61-3-9905 5613
email  [log in to unmask]