I am doing some labeling of tissues with two rabbit derived antibodies,
one against serotonin and one against a peptide called Tpep. I need to do
a double labeling experiment in order to determine whether both these
antigens reside in the same or different neurons and their axons.

Two questions

1. Can anyone suggest a reliable method for double labeling using a
secondary fluor-conjugated probe with two different antibodies derived
from the same species (rabbit)? I don't have a large supply of either of
these antibodies.

2. Is there anyone who would be interested in trading some goat-derived
serotonin antibody (IncStar/Diasorin) and appropriate secondary antibody
produced in an animal other than goat or rabbit (e.g. Donkey) for some
"Small Cardioactive Peptide" monoclonal antibody (mouse; Kempf, S.C., B.
Masinovsky and A.O.D. Willows. 1987. A simple neuronal system
characterized by a monoclonal antibody to SCP neuropeptides in embryos and
larvae of Tritonia diomedea (Gastropoda, Nudibranchia). J. Neurobiol. 18,
pp. 217-236) and appropriate secondary antibody?

Thanks,

Steve Kempf

_________________________________________________________________________
The mind is like a parachute; it works much better when it's open.
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Stephen C. Kempf                             Tel: 334-844-3924
Associate Professor                          Fax: 334-844-4065
Depart. of Zoology and Wildlife Science
101 Cary Hall
Auburn University, AL  36849
USA                                        Email: [log in to unmask]
Director - AU Hybridoma Facility - www.auburn.edu/research/hybridoma/

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