CONFOCALMICROSCOPY Archives

October 1999

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
CCD sensitivity // Re: 6 bits is plenty (CCD for fluorescence work)
From:
Milton Charlton <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 19 Oct 1999 12:45:23 -0400
Content-Type:
TEXT/PLAIN
Parts/Attachments:
TEXT/PLAIN (22 lines) 
It is possible to use cheap cameras like Cohu 3815 to integrate for
several frame times.  If you can not integrate and do not want to use a
noisy intensifier then I think you should look for a camera with the highest
quantum efficiency.  SITE back thinned chips have high QE and Kodak has
a new chip with ~85% QE called KAF1410E or similar.  Has anyone tried
these and found there is a usable increase in sensitivity for
fluorescence microscopy?

Milton Charlton

Milton P. Charlton
Professor
Physiology Department, Med.Sci. Bldg. 3232
MRC Group
University of Toronto
1 King's College Circle
Toronto, ON, M5S1A8
Canada
tel:416-978-6355
fax:416-978-4940
email: [log in to unmask]

ATOM RSS1 RSS2