CONFOCALMICROSCOPY Archives

October 1999

CONFOCALMICROSCOPY@LISTS.UMN.EDU

Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
From:
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 27 Oct 1999 17:40:17 EDT
Content-Type:
text/plain
Parts/Attachments:
text/plain (15 lines)
I may be a bit late responding, but have you considered caged compounds?
There are a number of chemicals available which are non-fluorescent until
they are hit with  UV.  The UV cleaves a piece off the molecule to release
the colored species.  Firing the laser into a cuvette a number of times, then
reading the released signal may be sensitive enough for what you want.

There aren't enough calibrated standards in microscopy.  I have worked on
developing in-house standards at our company, but keep looking for more
globally applicable solutions.

Good luck,

David
oQQQQ@

ATOM RSS1 RSS2