Confocalists,

I am interested in some feedback from list subscribers as to whether
fluorescence intensities in confocal images can be quantitated.  There seem
to be two schools of thought about this issue (with some "gray" in
between), one that does quantitation & wonders why I would raise this
issue, and another that is wary of performing quantitation on confocal
images due to the many difficult to control variables involved (optical,
staining, and sometimes inter-operator).

I would appreciate hearing under what conditions (if any) you would
consider quantitation to be a scientifically appropriate form of analysis.

I would also appreciate it if you could provide literature references that
would help me sort out this issue.

I am currently in the "wary" camp, but I'm trying to be open minded about
this.  I will post a summary of the replies to the list.

Yours,
Doug
.....................................................................
: Douglas W. Cromey, M.S.          Dept. of Cell Biology & Anatomy  :
: Research Specialist, Principal   University of Arizona            :
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