CONFOCALMICROSCOPY Archives

November 1999

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Beat Ludin <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 25 Nov 1999 10:06:13 +0100
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>  >
>  > 1. The SPOT camera is certainly a very good all-in-one choice.
>  >
>
>The main problem with the slow scan cameras (like the SPOT) is focussing
>with high NA lenses for weak fluorescence.

Aryeh, this is certainly true and I would also recommend the (slider
version of the) SPOT RT unless you need high sensitivity in the
orange to far-red in which case the SPOT-2E is definitely superior to
the SPOT RT, the DVC 1300, or any other interline camera on the
market, AFAIK.

However, Kathy was asking for a hi-res camera for H/E sections, so
focusing through the ocular should work fine as long as the camera is
properly parfocalized. Sensitivity is not an issue for this
application - but dynamic range and resolution are. In both respects,
the SPOT series should beat the DVC (36bit color vs. 30bit color,
1300x1000 true color vs. 1300x1000 interpolated color).

Beat


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