CONFOCALMICROSCOPY Archives

November 1999

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Subject:
inverted water immersion and heating
From:
Kees Jalink <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 26 Nov 1999 10:03:59 +0100
Content-Type:
text/plain
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text/plain (25 lines) 
Dear listmembers,

I have a problem that pops up while doing confocal physiology
experiments.
I use a 63x water immersion lens (because the cells are 30um thick) on
an inverted Leica TCS-SP. I  image these cells for extended periods of
time (1-2 hours, but I plan to go up to 48 hours) at 37 degrees. Both
the culture chamber and the objective are temperature controlled. The
problem: within 30 minutes, the water droplet on the lens has
evaporated.

Has anyone solved this problem, or ideas how to proceed? Does immersion
oil with same index as water exist?

Regards, Kees

--

Kees Jalink Ph.D.
The Netherlands Cancer Institute, dept. of Cell Biology H1
Plesmanlaan 121, 1066CX Amsterdam, the Netherlands
020-5121933 (tel)   / 020-5121944 (fax) / [log in to unmask] (email)
at home: Paulus Potterlaan 5, 2102CC Heemstede
0235-476047 / [log in to unmask]

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