CONFOCALMICROSCOPY Archives

November 1999

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Subject:
From:
Anders Liljeborg <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 26 Nov 1999 10:33:16 +0100
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Hello Jacques,

I have looked into the configuration files for a Noran Odyssey XL,
we have two 60x objectives, and here are the values for the size of
one pixel in microns:

LENS_X_CALIB 0.1337410
LENS_Y_CALIB 0.1339208

LENS_X_CALIB 0.1288660
LENS_Y_CALIB 0.1292000

These values are set by a calibration procedure for each objective, so
it is individual for each objective. Your objective might have a
slightly different magnification.

Best wishes
Anders
>>>>> "Jacques" == Jacques Paysan <[log in to unmask]> writes:

    Jacques> Dear Confocalists I'm urgently seeking an answer to the
    Jacques> following question:

    Jacques> With a Noran Odyssee confocal microscope, equipped with a
    Jacques> 60x objective, zoom at 1x, 640x480 resolution: How many
    Jacques> pixels per micron ? Or microns per pixel??

    Jacques> I would very much appreciate your help, since I can't get
    Jacques> the information otherwise (the microscope is 12,000 miles
    Jacques> away and Noran closed for a holyday).

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        Assoc. Prof. Anders Liljeborg
        Biomedical Physics and X-ray Physics
        Teknikringen 14, 4 tr.
        Royal Inst. of Technology
        SE - 100 44 Stockholm, Sweden
        Phone +46 8 790 7219
        Fax.  +46 8 205 609
        E-mail: [log in to unmask]
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