Anil-
If you're on a confocal, select the FITC filters w/ the excitation source
for the Texas red [HeNe].  Repeat for the TR and compare w/ singly imaged,
proper filters and proper excitation.  This may give you an empirical read
on your cross talk.
Take heart, even if there is cross talk there are many remedies through
image subtraction, PMT control, and laser power.

        -Ramin

-----Original Message-----
From: Anil kumar Sharma [mailto:[log in to unmask]]
Sent: Wednesday, September 05, 2001 8:54 AM
To: [log in to unmask]
Subject: FITC and Texas Red


Dear members,

I have taken some images of a sample stained with FITC aand Texas
Red,wherein I feel there is a considerable degree of colocalisation between
the two signals. I just want to be sure that there is no fluorescence cross
talk between these two signals. Can anybody who has experience with these
dyes please help me with this. Atleast the profiles of these dyes seem to
rule out any cross talk.

Regards

Anil Sharma