*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Hi Emma,
You are correct, histological sections can be a problem because as sections deform, alignment often requires transformation/rotation as well as translation. This recent paper in Traffic describes a new ImageJ tool that could provide what you need:
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0854.2011.01309.x/abstract
After alignment is resolved I agree that ImageJ/Fiji can handle 3d data presentation with little problem.
All the best,
Tim Feinstein
Sent from my iPad
On Nov 21, 2011, at 4:18 AM, Emma King <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi All,
>
> Thanks for all your messages. Louis described much better than I did what we plan to do "In fact you want to add multiple Z-series together in order to form one huge series"!
>
> We have Volocity (and obviously imageJ) to visualise 3D data and I believe we can add z-series together, on top of each other. My worry is we will have issues aligning structures in one z-stack, from one tissue section, with the same structures in the next z-stack/tissue section.
>
> I'll give it a go using the suggestions made and come back to you if I get stuck!
>
> Thanks again,
> Em
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of CONFOCALMICROSCOPY automatic digest system
> Sent: 19 November 2011 06:05
> To: [log in to unmask]
> Subject: CONFOCALMICROSCOPY Digest - 17 Nov 2011 to 18 Nov 2011 (#2011-88)
>
> There are 12 messages totalling 698 lines in this issue.
>
> Topics of the day:
>
> 1. 3D reconstruction of multiple, fluorescently labelled, sections (3)
> 2. RE 3D reconstruction of multiple, fluorescently labelled, sections (3)
> 3. Olympus Corp in financial trouble? (2)
> 4. (lots) more on Olympus
> 5. Cell viability assay
> 6. Fast two-photon imaging: Constant fraction discriminator and variable
> delay for Laser pulse-synch
> 7. Olympus Corp in financial trouble? We certainly hope not!
>
> ----------------------------------------------------------------------
>
> Date: Fri, 18 Nov 2011 08:39:57 -0600
> From: Emma King <[log in to unmask]>
> Subject: 3D reconstruction of multiple, fluorescently labelled, sections
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=3Dconfocalmicroscopy
> *****
>
> We would like to take serial sections (20 microns thick) of rat spinal=20=
>
> cord (up to 1cm long), label them with a bright and specific fluorescent=20=
>
> label, acquire images (we can use either a widefield or confocal=20
> microscope) and then reconstruct the data in to one, 3D structure.=20
>
> We have the sectioning, staining and acquisition parameters/options=20
> under control, but don't have any software capable of reconstructing=20
> the resultant images. Does anyone have any suggestions as to what=20
> software to use and where to get it from?=20
>
> Any help much appreciated.=20
>
> Cheers,=20
> Emma=20
>
> Advanced Microscopy Unit=20
> School of Biomedical Sciences=20
> Univeristy of Nottingham
>
> ------------------------------
>
> Date: Fri, 18 Nov 2011 09:51:43 -0500
> From: [log in to unmask]
> Subject: RE 3D reconstruction of multiple, fluorescently labelled, sections
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=3Dconfocalmicroscopy
> *****
>
> Hi Emma,
>
> In fact you want to add mutltiple Z-series together in order to form one=20
> huge serie. First, you will need a good computer and 2nd, I have done it=20
> using Huygens software (SVI).=20
> Also, I am pretty sure that there is a plugin In" Image J" that can do=20
> that. Do not forget to do that with a good computer!!!!
>
> Best regards,
>
> Louis
>
> Louis Villeneuve
> Associ=E9 de recherche - Microscopie confocale
> Institut de Cardiologie de Montreal- Centre de recherche
> 5000 est B=E9langer
> Montreal (Qc), Canada
> H1T 3C8
>
> 514-376-3330 ext 3511=20
> 514-376-1355 (fax)
>
>
>
> Emma King <[log in to unmask]>@LISTS.UMN.EDU=20
> Envoy=E9 par : Confocal Microscopy List <[log in to unmask]>
> 2011-11-18 09:39
> Veuillez r=E9pondre =E0
> Confocal Microscopy List <[log in to unmask]>
>
>
> A
> [log in to unmask]
> cc
>
> Objet
> 3D reconstruction of multiple, fluorescently labelled, sections
>
>
>
>
>
>
>
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=3Dconfocalmicroscopy
> *****
>
> We would like to take serial sections (20 microns thick) of rat spinal=20
> cord (up to 1cm long), label them with a bright and specific fluorescent=20
> label, acquire images (we can use either a widefield or confocal=20
> microscope) and then reconstruct the data in to one, 3D structure.=20
>
> We have the sectioning, staining and acquisition parameters/options=20
> under control, but don't have any software capable of reconstructing=20
> the resultant images. Does anyone have any suggestions as to what=20
> software to use and where to get it from?=20
>
> Any help much appreciated.=20
>
> Cheers,=20
> Emma=20
>
> Advanced Microscopy Unit=20
> School of Biomedical Sciences=20
> Univeristy of Nottingham
>
> ------------------------------
>
> Date: Fri, 18 Nov 2011 09:56:23 -0500
> From: Louis Kerr <[log in to unmask]>
> Subject: Re: 3D reconstruction of multiple, fluorescently labelled, sections
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Emma,
>
> Have you seen the work from Stephen Smith's Lab at Stanford with array tomography? This is a link to a Cold Spring Harbor Protocol.
>
> http :// cshprotocols . cshlp .org/content/2010/11/ pdb .top89.full
>
> Good luck,
> Louie
>
> ----- Original Message -----
> From: "Emma King" < emma [log in to unmask]>
> To: CONFOCALMICROSCOPY @LISTS. UMN . EDU
> Sent: Friday, November 18, 2011 9:39:57 AM
> Subject: 3D reconstruction of multiple, fluorescently labelled , sections
>
> *****
> To join, leave or search the confocal microscopy listserv , go to:
> http ://lists. umn . edu /cgi-bin/ wa ?A0= confocalmicroscopy
> *****
>
> We would like to take serial sections (20 microns thick) of rat spinal
> cord (up to 1cm long), label them with a bright and specific fluorescent
> label, acquire images (we can use either a widefield or confocal
> microscope) and then reconstruct the data in to one, 3D structure.
>
> We have the sectioning, staining and acquisition parameters/options
> under control, but don't have any software capable of reconstructing
> the resultant images. Does anyone have any suggestions as to what
> software to use and where to get it from?
>
> Any help much appreciated.
>
> Cheers,
> Emma
>
> Advanced Microscopy Unit
> School of Biomedical Sciences
> Univeristy of Nottingham
>
>
> --
>
>
>
> Louis Kerr
> lkerr @ mbl . edu
>
> Research and Education Support Coordinator
> Marine Biological Laboratory
> 7 MBL Street
> Woods Hole, MA 02543
> 508-289-7273
> 508-540-6902 (FAX)
> 508-292-0289 (Cell phone)
>
> VISIT OUR WEB SITES:
> www . mbl . edu
> www .courses. mbl . edu
>
> ------------------------------
>
> Date: Fri, 18 Nov 2011 16:51:30 +0100
> From: Tineke Vendrig <[log in to unmask]>
> Subject: Re: 3D reconstruction of multiple, fluorescently labelled, sections
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We use the Andor software for our 3D movies, works nice and good!
>
> Tineke Vendrig
>
> --
> Tineke Vendrig, ing
> technical engineer optical microscopy
> Delft University of Technology
> Bionano Science
> Kavli Institute of Nanoscience
> Lorentzweg 1
> 2628LJ Delft
> room F185
> Tel: +31 15 2789299
> Fax:+31 15 2781202
> email: [log in to unmask]
> mobile phone: +31 624341412
>
>
> 2011/11/18 Emma King <[log in to unmask]>
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> We would like to take serial sections (20 microns thick) of rat spinal
>> cord (up to 1cm long), label them with a bright and specific fluorescent
>> label, acquire images (we can use either a widefield or confocal
>> microscope) and then reconstruct the data in to one, 3D structure.
>>
>> We have the sectioning, staining and acquisition parameters/options
>> under control, but don't have any software capable of reconstructing
>> the resultant images. Does anyone have any suggestions as to what
>> software to use and where to get it from?
>>
>> Any help much appreciated.
>>
>> Cheers,
>> Emma
>>
>> Advanced Microscopy Unit
>> School of Biomedical Sciences
>> Univeristy of Nottingham
>>
>
> ------------------------------
>
> Date: Fri, 18 Nov 2011 12:28:12 -0500
> From: "Cammer, Michael" <[log in to unmask]>
> Subject: Olympus Corp in financial trouble?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=3Dconfocalmicroscopy
> *****
>
> "Japanese officials say that at least $4.9 billion is unaccounted for in a =
> financial scandal at Olympus<http://topics.nytimes.com/top/news/business/co=
> mpanies/olympus_corporation/index.html?inline=3Dnyt-org> and are investigat=
> ing whether much of that money went to companies with links to organized cr=
> ime."
> http://www.nytimes.com/2011/11/18/business/global/japanese-police-investiga=
> te-olympus.html?_r=3D1&scp=3D3&sq=3Dolympus&st=3Dcse
>
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist
> Skirball Institute of Biomolecular Medicine
> Lab: (212) 263-3208 Cell: (914) 309-3270
>
> </PRE>
> <html>
> <body>
> ------------------------------------------------------------<br />
> This email message, including any attachments, is for the sole use of the i=
> ntended recipient(s) and may contain information that is proprietary, confi=
> dential, and exempt from disclosure under applicable law. Any unauthorized =
> review, use, disclosure, or distribution is prohibited. If you have receive=
> d this email in error please notify the sender by return email and delete t=
> he original message. Please note, the recipient should check this email and=
> any attachments for the presence of viruses. The organization accepts no l=
> iability for any damage caused by any virus transmitted by this email.<br />
> =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
> =3D=3D=3D=3D=3D=3D=3D=3D
> </body>
> </html>
> <PRE>
>
> ------------------------------
>
> Date: Fri, 18 Nov 2011 12:18:55 -0600
> From: Johannes Schindelin <[log in to unmask]>
> Subject: Re: RE 3D reconstruction of multiple, fluorescently labelled, sections
>
> This message is in MIME format. The first part should be readable text,
> while the remaining parts are likely unreadable without MIME-aware tools.
>
> --Boundary_(ID_BmV8ySyi3ZWYFpVvpZWp+A)
> Content-type: TEXT/PLAIN; charset=ISO-8859-15
> Content-transfer-encoding: 8BIT
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> On Fri, 18 Nov 2011, [log in to unmask] wrote:
>
>> Emma King <[log in to unmask]>@LISTS.UMN.EDU
>> Envoyé par : Confocal Microscopy List <[log in to unmask]>
>> 2011-11-18 09:39
>>
>>> We would like to take serial sections (20 microns thick) of rat spinal
>>> cord (up to 1cm long), label them with a bright and specific
>>> fluorescent label, acquire images (we can use either a widefield or
>>> confocal microscope) and then reconstruct the data in to one, 3D
>>> structure.
>>
>>> We have the sectioning, staining and acquisition parameters/options
>>> under control, but don't have any software capable of reconstructing
>>> the resultant images. Does anyone have any suggestions as to what
>>> software to use and where to get it from?
>>
>> In fact you want to add mutltiple Z-series together in order to form one
>> huge serie. First, you will need a good computer and 2nd, I have done
>> it using Huygens software (SVI).
>>
>> Also, I am pretty sure that there is a plugin In" Image J" that can do
>> that. Do not forget to do that with a good computer!!!!
>
> If all you want to do is a volume rendering, there are indeed 2 ImageJ
> plugins to do the job: Volume Viewer (non-accelerated) and 3D Viewer (this
> one uses your graphics adapter's accelerated 3D rendering).
>
> However, if you need to have surface models, you might want to look into
> the TrakEM2 plugin. It is very powerful, therefore it takes some training
> before one can exploit its capabilities in full.
>
> If you do not want to bother to find all the bits and pieces required for
> these plugins to run, please feel free to download Fiji (http://fiji.sc/)
> which bundles ImageJ with a lot of plugins (including the 3 mentioned
> above).
>
> Ciao,
> Johannes
>
> --Boundary_(ID_BmV8ySyi3ZWYFpVvpZWp+A)--
>
> ------------------------------
>
> Date: Fri, 18 Nov 2011 11:02:21 -0800
> From: "Armstrong, Brian" <[log in to unmask]>
> Subject: Re: RE 3D reconstruction of multiple, fluorescently labelled, sections
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=3Dconfocalmicroscopy
> *****
>
> You could try Reconstruct from NIH Human Brain project =
> (http://synapses.clm.utexas.edu/tools/reconstruct/reconstruct.stm ).
>
> In my opinion, Amira, Imaris, and Volocity, are probably best at 3D =
> visualization tools.=20
>
> Cheers,
>
> Brian D Armstrong PhD
> Assistant Research Professor
> Light Microscopy Core=20
> Beckman Research Institute
> City of Hope
> Dept of Neuroscience
> 1450 E Duarte Rd
> Duarte, CA 91010
> 626-256-4673 x62872
>
> http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging=
> /Pages/default.aspx
>
>
> -----Original Message-----
> =46rom: Confocal Microscopy List [mailto:[log in to unmask]] =
> =
> On Behalf Of Johannes Schindelin
> Sent: Friday, November 18, 2011 10:19 AM
> To: [log in to unmask]
> Subject: Re: RE 3D reconstruction of multiple, fluorescently labelled, =
> sections
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa=3FA0=3Dconfocalmicroscopy
> *****
>
> On Fri, 18 Nov 2011, [log in to unmask] wrote:
>
>> Emma King <[log in to unmask]>@LISTS.UMN.EDU=20
>> Envoy=E9 par : Confocal Microscopy List <[log in to unmask]>
>> 2011-11-18 09:39
>>
>>> We would like to take serial sections (20 microns thick) of rat spinal
>>> cord (up to 1cm long), label them with a bright and specific
>>> fluorescent label, acquire images (we can use either a widefield or
>>> confocal microscope) and then reconstruct the data in to one, 3D
>>> structure.=20
>> =20
>>> We have the sectioning, staining and acquisition parameters/options
>>> under control, but don't have any software capable of reconstructing
>>> the resultant images. Does anyone have any suggestions as to what
>>> software to use and where to get it from=3F=20
>> =20
>> In fact you want to add mutltiple Z-series together in order to form one
>> huge serie. First, you will need a good computer and 2nd, I have done
>> it using Huygens software (SVI).=20
>>
>> Also, I am pretty sure that there is a plugin In" Image J" that can do=20
>> that. Do not forget to do that with a good computer!!!!
>
> If all you want to do is a volume rendering, there are indeed 2 ImageJ
> plugins to do the job: Volume Viewer (non-accelerated) and 3D Viewer (this
> one uses your graphics adapter's accelerated 3D rendering).
>
> However, if you need to have surface models, you might want to look into
> the TrakEM2 plugin. It is very powerful, therefore it takes some training
> before one can exploit its capabilities in full.
>
> If you do not want to bother to find all the bits and pieces required for
> these plugins to run, please feel free to download Fiji (http://fiji.sc/)
> which bundles ImageJ with a lot of plugins (including the 3 mentioned
> above).
>
> Ciao,
> Johannes
>
>
> ---------------------------------------------------------------------
> *SECURITY/CONFIDENTIALITY WARNING: =20
> This message and any attachments are intended solely for the individual or =
> =
> entity to which they are addressed. This communication may contain =
> information that is privileged, confidential, or exempt from disclosure =
> under applicable law (e.g., personal health information, research data, =
> =66inancial information). Because this e-mail has been sent without =
> encryption, individuals other than the intended recipient may be able to =
> view the information, forward it to others or tamper with the information =
> =
> without the knowledge or consent of the sender. If you are not the intended=
> =
> recipient, or the employee or person responsible for delivering the message=
> =
> to the intended recipient, any dissemination, distribution or copying of th=
> e=
> communication is strictly prohibited. If you received the communication in=
> =
> error, please notify the sender immediately by replying to this message and=
> =
> deleting the message and any accompanying files from your system. If, due t=
> o=
> the security risks, you do not wish to receive further communications via =
> =
> e-mail, please reply to this message and inform the sender that you do not =
> =
> wish to receive further e-mail from the sender.=20
>
> ---------------------------------------------------------------------
>
> ------------------------------
>
> Date: Fri, 18 Nov 2011 13:24:11 -0600
> From: Martin Wessendorf <[log in to unmask]>
> Subject: Re: Olympus Corp in financial trouble?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Michael et al--
>
> On 11/18/2011 11:28 AM, Cammer, Michael wrote:
>
>> "Japanese officials say that at least $4.9 billion is unaccounted for in a financial scandal at Olympus<http://topics.nytimes.com/top/news/business/companies/olympus_corporation/index.html?inline=nyt-org> and are investigating whether much of that money went to companies with links to organized crime."
>> http://www.nytimes.com/2011/11/18/business/global/japanese-police-investigate-olympus.html?_r=1&scp=3&sq=olympus&st=cse
>
> I've seen these reports, too, over the past few weeks. However,
> although it seems quite likely that their stockholders will be unhappy
> with them, it's unclear (to me, at least!) that the company is in
> financial trouble yet.
>
> Disclaimer: I'm definitely not a certified financial advisor!
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D. office: (612) 626-0145
> Assoc Prof, Dept Neuroscience lab: (612) 624-2991
> University of Minnesota Preferred FAX: (612) 624-8118
> 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009
> Minneapolis, MN 55455 e-mail: [log in to unmask]
>
> ------------------------------
>
> Date: Fri, 18 Nov 2011 15:26:43 -0600
> From: Martin Wessendorf <[log in to unmask]>
> Subject: (lots) more on Olympus
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> For anyone who's interested:
>
> http://newsfeedresearcher.com/data/articles_b47/olympus-woodford-company.html
>
> Martin
> --
> Martin Wessendorf, Ph.D. office: (612) 626-0145
> Assoc Prof, Dept Neuroscience lab: (612) 624-2991
> University of Minnesota Preferred FAX: (612) 624-8118
> 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009
> Minneapolis, MN 55455 e-mail: [log in to unmask]
>
> ------------------------------
>
> Date: Fri, 18 Nov 2011 14:34:04 -0800
> From: Iain Johnson <[log in to unmask]>
> Subject: Re: Cell viability assay
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Calcein AM (intracellular esterase substrate). Incubate cells for 10
> minutes in 1 microM calcein AM in serum-free medium at 37C. Wash off
> staining medium and replace with complete culture medium. Image in
> FITC/GFP channel.
>
> You can also add a second dimension to the assay (at the expense of giving
> up more spectral real estate).
>
> SYTOX Orange (Molecular Probes; membrane permeability probe). Add to
> complete culture medium at 5 microM. Do not wash. Image in TRITC/Cy3
> channel.
>
> Iain
>
> Iain Johnson Consulting
> Eugene, OR
> (541) 285-8296
>
> On Thu, Nov 17, 2011 at 7:23 AM, B. Prabhakar Pandian <[log in to unmask]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
>> *****
>>
>> Hello,
>> I am emailing to see if anybody has a suggestion for a cell
>> viability/toxicity assay that works similar to LDH/Caspase but relies on
>> internal enzymes rather than in the supernatant. In addition, it should be
>> compatible with fluorescence microscope.
>>
>> Thanks,
>>
>> -Prabhakar
>>
>
> ------------------------------
>
> Date: Fri, 18 Nov 2011 19:50:53 -0500
> From: Christian Wilms <[log in to unmask]>
> Subject: Re: Fast two-photon imaging: Constant fraction discriminator and variable delay for Laser pulse-synch
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Regarding the delay: if you do not need it to flexible in timing,
> simply getting a coaxial cable of the correct length (20 cm
> corresponds roughly to 1 ns) is one option. A bit more flexible and
> still affordable are variable delay lines such as the Ortec 425 <http://www.ortec-online.com/download/425A.pdf
>
> Best, Christian
>
> ------------------------------
>
> Date: Fri, 18 Nov 2011 23:08:24 -0500
> From: "Alison J. North" <[log in to unmask]>
> Subject: Re: Olympus Corp in financial trouble? We certainly hope not!
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=3Dconfocalmicroscopy
> *****
>
> Hey Michael,
>
> There is no question that Olympus has been going through hell this year - s=
> tarting with the tsunami, and who could have guessed that things could have=
> got even worse because of a few crooks (worst case) or idiots (best case) =
> at the top.... Given that we own several high-end Olympus microscopes, we=
> obviously have a vested interest in the company staying afloat and are und=
> erstandably all concerned. More than that though, I have worked with the s=
> ame Olympus team for over 11 years now and they have not only proved to be =
> great colleagues but also good friends - we have caught wild mice on our ha=
> nds and knees in my lab together, I have played despicable April Fool's jok=
> es on them (well truthfully, only once - they have refused to visit my lab =
> on April 1st ever since), and they have proved themselves such wizards at s=
> olving the most mysterious and intractable microscope problems that we nick=
> named the two local guys Dumbledore and Gandalf. At this point I think any=
> discussion of what will happen is just pointless speculation, and one whic=
> h they cannot possibly enter into, so the best we can do is to trust this w=
> ill all blow over eventually and offer the microscope guys our support, say=
> ing: we're rooting for you, and here's to 2012 being a far better year for =
> you than 2011!
>
> Best,
> Alison
> P.S. Sorry if I'm gushing to the confocal listserver - blame the glass of =
> wine in my hand and the fact that I just came back from the movies :-).=20
>
> =20
> ________________________________________
> From: Confocal Microscopy List [[log in to unmask]] On Behalf=
> Of Cammer, Michael [[log in to unmask]]
> Sent: Friday, November 18, 2011 12:28 PM
> To: [log in to unmask]
> Subject: Olympus Corp in financial trouble?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=3Dconfocalmicroscopy
> *****
>
> "Japanese officials say that at least $4.9 billion is unaccounted for in a =
> financial scandal at Olympus<http://topics.nytimes.com/top/news/business/co=
> mpanies/olympus_corporation/index.html?inline=3Dnyt-org> and are investigat=
> ing whether much of that money went to companies with links to organized cr=
> ime."
> http://www.nytimes.com/2011/11/18/business/global/japanese-police-investiga=
> te-olympus.html?_r=3D1&scp=3D3&sq=3Dolympus&st=3Dcse
>
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist
> Skirball Institute of Biomolecular Medicine
> Lab: (212) 263-3208 Cell: (914) 309-3270
>
> </PRE>
> <html>
> <body>
> ------------------------------------------------------------<br />
> This email message, including any attachments, is for the sole use of the i=
> ntended recipient(s) and may contain information that is proprietary, confi=
> dential, and exempt from disclosure under applicable law. Any unauthorized =
> review, use, disclosure, or distribution is prohibited. If you have receive=
> d this email in error please notify the sender by return email and delete t=
> he original message. Please note, the recipient should check this email and=
> any attachments for the presence of viruses. The organization accepts no l=
> iability for any damage caused by any virus transmitted by this email.<br /=
>>
> =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
> =3D=3D=3D=3D=3D=3D=3D=3D
> </body>
> </html>
> <PRE>
>
> ------------------------------
>
> End of CONFOCALMICROSCOPY Digest - 17 Nov 2011 to 18 Nov 2011 (#2011-88)
> ************************************************************************
> This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham.
>
> This message has been checked for viruses but the contents of an attachment
> may still contain software viruses which could damage your computer system:
> you are advised to perform your own checks. Email communications with the
> University of Nottingham may be monitored as permitted by UK legislation.
|
