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Date: | Fri, 26 May 2006 10:20:34 -0700 |
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Clever little bugger, aren't you?
Could you communicate something to Kirsten for me, when it seems
appropriate? I've got about 28 copies of Alan's book that I was
going to take up to UBC for him. Will take inventory tonight.
Does she want to handle credit cards and PO numbers, stick with cash?
I'm sure there are many other issues that are more important to her
right now.
Regards,
Glen
On May 25, 2006, at 6:08 PM, Stephen Cody wrote:
> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-
> bin/wa?S1=confocal Search the CONFOCAL archive at http://
> listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Dear Siva,
>
>
> You can make a very simple water jacket to go around your line from
> the peristaltic pump, to keep it warm until it reaches your cell
> bath. All you need is a laboratory water heater/pump, some tubing
> of different sizes and two “y-connectors” and 10 minutes assembly
> time.
>
>
> The tubing to run the saline from your pump to the cell bath should
> be a lot thinner diameter of the tubing to act as a water jacket.
>
>
> Run this thin tubing inside the water jacket tubing. The water
> jacket tubing should be a little shorter than the distance from
> your pump to the microscope. One end of the thin tubing should be
> fed though one arm of one “y-connector”. The y–connector can then
> be attached to the water jacket thick tubing. This process should
> be repeated with the other end. Where the thin tubing emerges from
> the y-connectors should be sealed with thick walled silicon tubing.
> The remaining ends of the y-connectors should be attached to tubing
> to connect to the recirculating water bath. The flow should
> preferably be counter current. And because none of this makes any
> sense you can download a diagram here:
>
>
> www.ludwig.edu.au\confocal\cody\Heat_exchange.jpg
>
>
> Happy plumbing.
>
> Stephen H. Cody
>
> Microscopy Manager
> Central Resource for Advanced Microscopy
> Ludwig Institute For Cancer Research
> PO Box 2008 Royal Melbourne Hospital
> Parkville Victoria 3050
> Australia
> Tel: 61 3 9341 3155 Fax: 61 3 9341 3104
> email: [log in to unmask]
> www.ludwig.edu.au/labs/confocal.html
>
>
>
>
>
> This communication is intended only for the named recipient and may
> contain information that is confidential, legally privileged or
> subject to copyright; the Ludwig Institute for Cancer Research does
> not waiver any rights if you have received this communication in
> error.
> The views expressed in this communication are those of the sender
> and do not necessarily reflect the views of the Ludwig Institute
> for Cancer Research.
>
>
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