Thank you Steve,

still I don't know, if with Leica TCS Bitplane mean the (old) TCS 4d,
TCS NT or TCS SP. I would guess that they mean the 4D. Or do they all
have the same relation digital pinhole <-> physical pinhole size? Does
anybody know?

Sincerely, Joachim

Steve Bagley wrote:
>
> Hi,
> I don't know how much use this is for your specific problem but if you
> visit the Bitplane website (www.bitplane.com) and then head over to
> the support section there is a Pinhole look up table for 63 and 100x
> magnification. The input values (objective magnification, machine
> number) are converted to the size of the pinhole back projected into
> the sample plane (in nm) and can be used for deconvolution purposes.
> This has been calculated for both Leica (TCS)  and Zeiss (310,410 and
> 510) instruments.
> Hope this has helped,
> Steve
>
> Steve Bagley
> CRC Structural Cell Biology Group,
> Paterson Institute for Cancer Research,
> Christie Hospital,
> Manchester M20 9BX, UK
> http://www.paterson.man.ac.uk
>
> Joachim Walter wrote ...
>
> Hello Leica users,
>
> for my experiments I need to know the size of the pinhole of the Leica
> TCS SP backprojected onto the sample. The software shows me a digital
> pinhole size of 90 and claims this to be the size of one Airy disk
> (Objective: 100x/1.3). However, I doubt this, as the Airy disk reading
> doesn't change with the chosen excitation/emission wavelength. Maybe
> Leica calculates the Airy disk reading for a fixed reference
> wavelength?  Can anybody help me?
> Thank you in advance,
> Joachim

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Joachim Walter, Dipl. Phys.
Institut für Anthropologie und Humangenetik der LMU München
AG Cremer
Richard-Wagner-Straße 10/I
D-80333 München               Tel. +49 - 89-2180-6713
Germany                       Fax. +49 - 89-2180-6719
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