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Maybe I am missing something here, but how about just adding glycerol to 
the solution? Other options might be a low concentration of 
low-melting-point agarose or a gel used for ultrasonic examinations.

Steffen

On 28.06.2013 21:08, Jen Jackson wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Hello all,
>
>
> I'm looking for a method to 'slow down', but not completely immobilize- a
> low concentration of YFP particles to help characterize a TIRF system.
> Ideally, we could perhaps later use this "YFP gel" to calibrate fluorescence
> intensity of single YFP molecules in biological membranes, but for now I'm
> looking for a decent way to have some sort of reduction in particle speed,
> as compared to solution, without compromising the spectral properties (etc.)
> of the protein.  Any tips, tricks?
>
>
> Many thanks,
>
> Jen
>


-- 
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

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