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yes, there are all kinds of led,different wavelength, 375nm,405nm,460nm,585nm,....and the optical power is high, maybe up to 100mw. I have ever bought different leds, for a 5w blue led(460nm),the optical power is about 20mw(it is easy to adjust the power by changing the current or voltage ),the price is about 14 RMB in china (about 2 dollar).Compare with laser, the biggest problem is led's big emission angle.So it is hard to couple light to fiber or focus into one point.I tried to do it, but most of the energy lost.
I think the led will replace the arc lamp in future, even the laser for confocal or laser scanning microscopy.
Date: Wed, 7 Nov 2007 15:03:44 -0500From: [log in to unmask]: Re: LED price ?To: [log in to unmask] the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi Barbara,
is there a broader choice of the excitation wavelength, e.g. is there 430 nm (440 nm), 570 nm (or 580 nm) LEDs? Let say matching the 89006 ET set from Chroma?
Vitaly
NCI-Frederick,
301-846-6575
----- Original Message -----
From: Barbara Foster
To: [log in to unmask]
Sent: Wednesday, November 07, 2007 1:11 PM
Subject: Re: LED price ?
Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi, JeremyThe price is for an LED cassette, which includes intelligent electronics. Since each LED requires a specific voltage to drive it, the ability for the system to sense which LED cassette has been inserted is critical, especially for 2-channel or 3-channel imaging. And yes, you would have to buy several LED cassettes. However, when you consider that the lifetime is in excess of 30,000 hrs (I spoke to a diagnostic company yesterday who OEMs this system and they told me that, in practice, it was often in excess of 50,000 hrs) and there is often a better S/N ratio, it's not a very big investment compared to a mercury arc.Hope this was helpful,Barbara Foster, PresidentWe've moved! Microscopy/Microscopy Education7101 Royal Glen Trail, Suite AMcKinney TX 75070P: (972)924-5310Skype: fostermmeW: www.MicroscopyEducation.comMME is now scheduling customized, on-site courses through December.
Call us today for details.P. S.Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Just call us here in the MME office for details. At 05:06 AM 11/7/2007, you wrote:
Search the CONFOCAL archive athttp://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalre the informative posting on LEDs by Barbara Fostercatalogue prices for LEDs seem to be very low, so how comeCost of LED cassette: Eu720 ? which seems to be a couple of orders of magnitude greater.In addition you would need to purchase several LEDs Jeremy AdlerCell BiologyThe Wenner-Gren Inst.Arrhenius Laboratories E5Stockholm UniversityStockholm 106 91Sweden-----Original Message-----From: Confocal Microscopy List on behalf of Barbara FosterSent: Tue 06/11/2007 17:27To: [log in to unmask]: Re: Non-arc source for IX-81 - semi commercial Search the CONFOCAL archive athttp://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalDear GlenAs a strategic consultant in microscopy, I get to see the latest technology and there is, indeed, a great deal of flurry about LED technology. In the summer of 2006, I had a chance to evaluate the AFTER/FluoLED from Fraen and was very impressed
with the design, ease of use, and flexibility. I have been working on assignment with Fraen more recently and was surprised to see how much both LED technology and this product line had evolved. So here are observations on both LED technology in general, and the Fraen system in particular.Fraen's FluoLEDs are now available in UV (354nm), Royal blue (450nm), Blue (480nm), Cyan (505 nm), Green (535nm) Yellow (590nm) and red (630nm). While Fraen is a new name in the microscopy arena, most of you already know them: they are the world's largest manufacturer of the LEDs used for the pointers/indicators for the speedometers, gas gauges, etc., on the dashboard of your cars.Until recently Fraen's AFTER/FluoLEDs were only available in transmitted light version for upright microscopes, currently, over 17 different models from all the major manufacturers and several of the smaller ones. For us "old timers", transmitted light has typically been seen as less efficient, but the superb
images from FluoLED tell a very different story: Bright features against wonderfully velvet black background. In other words: great S/N. Fraen will be releasing the first systems for inverted stands next month and have begun work on an epi version as well.As with any technology, there is up side/down side to LEDsThe good news is the consistency, lack of fuss, and economy of LEDs. When they are on, they are on. When they are off and you need them on, you can turn them on immediately - no cycle time.Also, they exhibit much less drop off over time than HBOs. That time factor is critical. Life expectancy of an HBO is on the order of 200-300 hrs; for Fraen's LED's (I don't have figures on the others) 30,000 hrs. No error in decimal points here: you can run them 8 hrs a day, 5 days a week, for 5 years without changing a lamp. If you plot drop-off versus time, a 100 fold increase in time is significant, especially for those of us doing long term experiments.When it comes t
ime to switch out the lamp, there is no alignment, no disposal issue.The economy issue is also an interesting. Fraen's European office did the following calculations (Euros) for the LED cassette for a standard Blue excitation kit vs. an HBO arc lamp:Cost of LED cassette: Eu720 Cost of HBO lamp: 160Lifetime LED casette: 30,000hrs Lifetime HBO lamp: 300 hrsEu/hr LED cassette: EU 0.024 Eu/hr HBO lamp: Eu 0.53Assumption: if you run both systems for 2000 hrs/yearCost of LED cassette/yr: Eu48 Cost of HBOs/year: Eu1060.Savings, using LEDs: Eu1012One more bit of good news: LEDs are also a much cooler source so there is dramatically less photobleaching.The down side really isn't very down, just something to be aware of.Because of the state of LED technology, green and yellow LEDs generate less power so the resulting images will be somewhat less bright than with HBO. This is not much of an issue when the fluorescence is viewed at magnifications up t
o about 60x but if you routinely use 100x objectives, you should run the test to see if it is a problem with your particular samples. The good news is (a) for green LEDs, research is powering ahead. Fraen expects to have new, brighter LEDs in Feb 08. (b) For Yellow (Texas red, etc.), research is slower. However, they also have a good news side: they exhibit better S/N ratio, even at the lower power, than HBO.The FluoLED family has a number of things to recommend it:a. They have engineered a clever "multi-cube" device so that you can have 1 LED, 2 LEDs, or 3 LEDs and can switch conveniently from one to anotherb. For multi-user labs, the LED cassettes can be switched quickly and easily. This feature reminded me of the old Reichert Polyvars, one of my favorite microscopes, especially for teaching. The fluorescence (and reflected light DIC and Darkfield) cubes came on "lolly pop" sticks so that you could just slide in what you needed. FluoLED has mimicked that flexibility
with their cassette approach. A lab can have a set of cassettes sitting in a drawer next to the microscope or each group can have what they need in their own area, so they can have whatever excitation/emission they need by just plugging in their cassette and tightening the locking screw. Immediate change out... no alignment!c. Fraen has engineered intelligent electronics into their controllers. Different wavelength LEDs require different amperages to drive them. With Fraen's system, when a cassette is plugged into position, the controller intelligently senses which LED is in the cassette and provides the appropriate amperage, even with the 3 cassette system.d. The controller also allows the user to change intensity so that you can balance different channels for optimum imaging.e. Finally, and as a past high school teacher, I loved this one... Fraen has engineered less expensive "baby" systems in Blue and Royal blue, so that we can finally get fluorescence into teaching la
bs.That's the story. I hope it was helpful. I am at Neuroscience this week and LEDs are, indeed,grabbing a lot of interest.Best regards,Barbara Foster, PresidentWe've moved!Microscopy/Microscopy Education7101 Royal Glen Trail, Suite AMcKinney TX 75070P: (972)924-5310Skype: fostermmeW: www.MicroscopyEducation.comMME is now scheduling customized, on-site courses through December. Call us today for details.P. S.Need a good general reference or light microscopy text for next semester? Call us today to learn more about "Optimizing LIght Microscopy". Copies still available through MME... even for class-room lots ... and we give quantity discounts. Just call us here in the MME office for details.At 07:21 AM 11/6/2007, Gerard Whoriskey wrote:>Search the CONFOCAL archive at> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal>>Hi Glen,>The argument for LED systems is very strong on reliability and operational>costs and is continually improving with regard to performance, measu
red in>choice of wavelengths and intensity.>I assume that in your confocal set-up you are only using the mercury based>bulb system to check and align samples and that you only need excitation>regions that match the laser lines you are using. An LED system that you>can switch on and off as you please is ideal for such applications and a>very cost effective replacement to bulbs.>Commercial bit:>We have only recently included 445nm and 505nm options to our range. Now>users can choose from 7 options of 400nm, 445nm, 465nm, 505nm, 525nm,>595nm, and 635nm.>I will contact you directly with more commercial information.>>Best Regards,>>Gerry>>Gerard Whoriskey>Development Engineer>CoolLED Ltd>CIL House>Charlton Road>Andover>Hampshire>SP10 3JL>>Mob: 07789535762>Tel: +44 (0) 1264 321321>Dir: +44 (0)1264 320984>web site: www.coolled.com
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