Judy Drazba wrote:
>
> Could someone recommend an anti-fade mountant for use with DiI/DiO labelled
> cells.  The usual glycerol-based mounting media cannot be used because the
> dyes "bleed." Molecular Probes recommended something called Cytoseal.  Has
> anyone tried it?  Any other suggestions?
>
> TIA
> Judy
>

I have also had this problem of "bleeding" with DiI and DiO and is
currently looking for a way to mount and clear stained preps.  I have
also used glycerol as well a radiopaque contrast medium called Hypaque
at someone's reccomendation, but also encountered the problem of
bleeding.  I would also appreciate any suggestions on this matter.

Thank you

Francine Lewis ([log in to unmask])
Dept. of Biology,
McGill University
1205 Avenue Dr. Penfield
Montreal PQ H3A 1B1
Canada