PFA does not kill GFP fluorescence.

BAD PFA kills GFP fluorescence.

We routinely use 1% PFA with GFP, YFP, and CFP, and have never had a problem
so long as the PFA is fresh and pH¹ed to between 7.0 and 7.4.

    -Steve



On 4/22/09 10:34 PM, "Young Jik Kwon" <[log in to unmask]> wrote:

> All,
> 
> We are trying to take confocal micrographs of at tumor tissues that express
> GFP. What would be the sample preparation procedures? We tried cryosectioned
> samples but the cell morphology seems weird. We already know paraformaldehyde
> fixation kills GFP fluorescence. Any expert's advice?
> 
> Best,
> 
> Young
> 
> 
> 


****************************************************************************
Stephen C. Bunnell, Ph.D.
Assistant Professor
Tufts University Medical School
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