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Hi,

We have found that our images get lines in them, from laser fluctuations
if the laser overheats, especially the Krypton.  If the AC is set above
69 degrees F we see problems.

Deb Berglund
Montana State University

Empson R wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear All
>
>I'd like some advice if possible ...
>
>We have a Bio Rad Radiance 2100 which we are really pleased with, we have
>had it nearly two years now. In the UK unlike USA, air conditioners are not
>very reliable and ours keeps breaking. Although we have a fan that ducts air
>from the back of the ICU out of the room I am very concerned that the
>cooling without the air conditioning will not be sufficient.
>
>Without the air con the room goes to around 22-24 C, (with the air con it
>stays around 19), 24C seems too high for safe running of the lasers so I am
>concerned about their life.
>We have an Argon - the one I am most worried about, and 2 diode lasers and
>Green He/Ne and Red.
>
>I am trying to get support from our Estates service to replace the unit.
>
>Any comments would be very useful
>
>Ruth
>
>Dr Ruth M. Empson
>Lecturer in Neuroscience
>School of Biological Sciences
>Royal Holloway University of London
>EGHAM
>Surrey
>TW20 0EX
>
>Tel 01784 414264
>Digital voicemail 07092 076899
>Fax 01784 434326
>
>*       To find out more please visit the lab homepage at
>http://web.rhul.ac.uk/Biological-Sciences/empson/
>
>
>
>>-----Original Message-----
>>From: Ran Evans [SMTP:[log in to unmask]]
>>Sent: 11 June 2003 22:16 PM
>>To:   [log in to unmask]
>>Subject:      Confocal purchase
>>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>I would appreciate opinions on a possible confocal purchase.  Our core
>>facility
>>has 5 flow cytometers and 1 Olympus FV500 confocal.  We have a very
>>limited
>>budget of approximately $110,000 to $120,000 to spend on a second confocal
>>microscope.  Does anyone have suggestions on what to look into?  Is there
>>very
>>much out there in this range?  I would prefer it to be as live-cell
>>friendly as
>>possible, but this is not absolutely necessary.  Please contact me on or
>>off-line with any suggestions.
>>
>>Thank you,
>>
>>-Ran
>>
>>Randall Evans, Ph.D.
>>Flow Cytometry and Image Analysis Core Facility
>>MDAnderson Cancer Center
>>Houston, Tx  77030
>>(713)-723-8225
>>[log in to unmask]
>>
>>
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