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> Can anyone recommend a software or method to normalize image
> brightness for a confocal
> image series? We have been collecting large stacks of images (about
> 100 images/stack)
> and photo-bleaching progressively reduces the brightness of images.
> We hope that we can
> normalize these images before reconstructing them in 3D.
>
i use bleach_cor, part of the xcosm package. requires unix/linux
system. but works quite fine for me. using the same algorithm
(non-linear regression.. fairly straightforward i guess) should be
possible to write a imagej plugin. anybody did already?
tobias