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Hi Axel,

We tried several methods and the one developed by Dr. Kota Miura as a
script for IgorPro worked the best for us. It has a very nice
algorithm compensating for general photobleaching (outside of the ROI
of interest), has more flexibility in fitting parameters, offers three
different ways to fit the data.
The procedure file with the detailed introduction can be found here:
http://cmci.embl.de/downloads/frap_analysis

Best wishes,
Vladimir


===============
Vladimir Ghukasyan
Confocal and Multiphoton Imaging Facility
Neuroscience Center

115 Mason Farm Rd., Bld. 245, Rm. 7109F
Chapel Hill 27599-7250
NC

Tel.: (919) 966 5807
Fax: (919) 966 9605

On Tue, Nov 22, 2011 at 4:14 AM, Axel Kurt Preuss (IMCB)
<[log in to unmask]> wrote:
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>
>
> Hi everybody
> Would anybody know a freeware or online software tool, simple to use, which calculates the half time of FRAP curves
>
> Thanks
> Axel
>
>
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