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Invitrogen's Live/Dead assay includes calcein, a dye that is only taken up by live cells and it only becomes fluorescent when it is exposed to cytoplasmic esterases.  It's a green dye and I am pretty sure that you can purchase it separately and not just as part of the kit.
Doug

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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Eric Shelden
Sent: Wednesday, September 17, 2014 10:49 AM
To: [log in to unmask]
Subject: General fluorescent stain for cytoplasm

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All:

I am looking for a way to fluorescently counter-stain the volume of cultured cells, with a result similar to what would be achieved by having them express GFP in the cytoplasm. I have seen some references to staining with eosin Y. Does anyone have other suggestions?

Thanks in advance.

Eric