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Ask simon Watkins at pitt.  He was discussing this recently but I forget wha
he said

Clare M. Waterman-Storer, Ph.D.
Associate Professor
Laboratory for Cell Motility Studies
Department of Cell Biology CB163
The Scripps Research Institute
10550 North Torrey Pines Road
La Jolla CA, 92037 USA
Office:  858-784-9764
Lab:  858-784-9243
Secretary (Lorraine Lathrop): 858-784-9964
Fax:  858-784-9779
Email:  [log in to unmask]

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Maher, Robert
Sent: Wednesday, May 11, 2005 11:30 AM
To: [log in to unmask]
Subject: DRAQ-5 and tissue sections

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Does anyone have any experience with labeling sections with the nuclear
probe DRAQ-5 (BioStatus Ltd.)?  I'm specifically interested in the mounting
medium chosen.  I labeled tissue sections with DRAQ-5 and mounted the
sections with ProLong Gold (Molecular Probes).  After a day the staining is
significantly decreased.  I've also mounted the sections in water and
glycerin and have had somewhat better success.  Any insight would be
appreciated.

Robert Maher, Scientist
Safety Sciences Michigan-Ann Arbor
Pfizer Global Research and Development
e-mail:  [log in to unmask]




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