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I don't use any nail polish or any sealant. Unless you plan to keep your slides for a long time it isn't necessary and you won't risk ruining your slides with nasty nail polish. If keeping the slides is critical try some of the many "hard-set" mountants on the market for fluorescence.

----- Original Message -----
From: Guillermo Palchik <[log in to unmask]>
Date: Thursday, November 16, 2006 11:44 am
Subject: Substitute to Nail Polish

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Dear Confocalists,
> 
> Does anybody have any suggestions as far as what should I use to 
> secure 
> my coverslips to slides? I do not want to use nail polish anymore!
> 
> Gil
>