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Hi again Gabor,

Yes, the CompuCyte laser scanning cytometer (LSC) and iCys and iCyte 
imaging scanning cytometers, now part of ThorLabs,
https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=7473
https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=7676

routinely acquired transmitted light data through the condenser, and in 
the case of the "i" instruments, produced an image.

The Huron Digital Patyhology (previously Huron Technologies, and 
previous to that Biomedical Photometrics Inc) TissueScope product line 
is typically both brightfield and confocal fluorescence. Many of the 
TissueScope products can use a large stage enabling scanning an entire 
adult human brain slice.
http://www.hurondigitalpathology.com/solutions/tissuescope-cf/

Several research groups have replaced the condenser lens (which is often 
as cheap a lens as the big four can get away with) with an objective 
lens. I first saw this in the mid-1980s by David Soll and his group at 
University of Iowa - to enable tracking live cell morphology details in 
higher time resolution by imaging both up and down.

More recently, 4pi, 4pi-STED, and iPALM are dual objective lenses facing 
each other. Some SPIM/DSLM systems also have face-to-face lenses, along 
with a third, and (if I recall correctly) in some configurations fourth, 
perpendicular.

Enjoy,

George


On 6/23/2015 5:27 AM, Csúcs  Gábor wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> On laser scanning confocals it is an often used imaging method to record a transmitted image via the condenser. My question is whether anyone of you used the same approach to record a "normal" wide-field transmitted image (e.g. parallel to the fluorescent one). If yes, could you please share your experience?
>
> Thanks        Gabor
>
>    


-- 



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/42