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I constructed a DODT system a number of years back using an oil-immersion
condenser. As long as you have a high NA condenser you can do all kinds of
imaging with it. We used a 785nm LED array for illumination and a similar
band-pass filter in front of the camera for imaging. It was a very easy
modification since many systems already include a 785nm filter for the
camera and the LED and DODT gradient generator can be attached to any ready
lamp port.

Craig

On Tue, Jun 23, 2015 at 7:23 AM, James Kerin <[log in to unmask]>
wrote:

> Dear Gabor,
> We routinely do this for widefield imaging or, photometry (using a
> photomultiplier to record fluorescence from an optically defined ROI).
> &nbsp;If I understand your question correctly then you need a red or
> infrared filter in line with the transmitted light source and a second
> camera port, filter wheel or image splitter device so as to record the
> red/ir transmitted alongside the fluorescence wavelengths. Typical
> configurations would be:&nbsp;
> (a) simultaneous transmitted/fluorecence using a dichroic beamsplitter to
> divert light between sensitive (fluorescence) and cheap (transmitted)
> cameras(b) sequentially with fluorescence using an automated filter wheel
> to change emitters(c) in parallel with fluorescence on your imaging camera
> using one channel of an image splitter for the transmitted light and the
> other(s) for fluorescence
> The main thing is to choose a transmitted wavelength that doesn't hurt
> your cells or overlap with the required fluorescence; and that will pass
> through the microscope dichroic. &nbsp;We have a range of dedicated
> products to achieve the configurations above and would be very happy to
> advise further
>
>
>
>
>
>
>
>
> J. P.  Kerin
> Marketing Director
> Cairn Research  Ltd
> Graveney Road
> Faversham
> Kent, ME13 8UP
> UK
>
>
> Tel: + 44 (0)1795 594507
> &nbsp;&nbsp;filters and mirrors now in stock!
>
>
> &nbsp;
>
>
>
>
> On  06/23/2015, 11:27am, Cs&uacute;cs  G&aacute;bor (
> [log in to unmask]) wrote:
> *****
>
> To join, leave or search the confocal microscopy listserv, go to:
>
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>
> Post images on http://www.imgur.com and include the link in your posting.
>
> *****
>
>
>
> Dear All,
>
>
>
> On laser scanning confocals it is an often used imaging method to record a
> transmitted image via the condenser. My question is whether anyone of you
> used the same approach to record a "normal" wide-field transmitted image
> (e.g. parallel to the fluorescent one). If yes, could you please share your
> experience?
>
>
>
> Thanks        Gabor
>