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Dear Mike,

Yes,You are right.The reseaon that I use beads is I have embeded beads
inside my sample so by measuring them in the different depths I can
have a good scale of being in the focal plane and then measuring the
SNR.

regards
sarah

On Thu, Apr 10, 2008 at 5:30 PM, MODEL, MICHAEL <[log in to unmask]> wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Maybe I am misunderstanding your question, but if you are interested in
> SNR why do you need beads? Cannot you just measure SNR for a comparable
> signal coming from something larger than a bead?
>
> Mike Model
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of Sarah Kefayati
> Sent: Thursday, April 10, 2008 4:41 PM
> To: [log in to unmask]
> Subject: SNR
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>
> Dear all,
>
> I appreciate if you help  me to figure out how I can calculate the SNR
> from the data that I obtain by imaging the fluorescent beads in
> different depths.
>
> Thanks
> Sarah
>