Confocal listservers,
I have a user who is using a 10x objective to image nerve tracks in brain
tissue. He is using an aqueous mounting media which completely dries. On some
preps he gets what looks like moire lines across his image. I have seen this
before and thought it maybe caused by drying artifacts between coverslip and
sections (which are picked up on the slide not the coverslip). Does anyone
have any suggestions which precludes the formation of these moire? lines and
an explanation of their formation. Also, they seem to be wavelenght dependent:
more pronounced with the 543 line than the 488 line. Is there a relation with
excitation or emission?

Hank Adams
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx

Hank Adams
Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030