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May 2004

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From:
"Edward Monosov, Ph.D." <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 4 May 2004 15:22:04 -0700
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Search the CONFOCAL archive at
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Hi Steve,
Here is a copy of my own answer to Gabor related to my setup:
"I am running PE Ultraview spinning disk under MM (v.6.1.5) using Multi
Dimensional Acquisition (MDA) module, which controls:
a) all firmware of the inverted Olympus IX81 microscope including the
Z-motor, nosepieces, filter cubes and condenser;
b) the Sutter Lambda 2-10 controller for the excitation and emission
filter wheels, and for the Uniblitz shutter installed into the bright
field illuminator.
c) the Orca ER (Hamamatsu) CCD camera.
d) the X-Y stage (ASI);
All these elements are hooked up to the left side port of my Olympus; "

On the right side port  I have another optical setup included the
"Dual-View" (Optical Insights), CCD camera "Cascade" (Roper) and DJ-4
(Sutter). Everything  controls.by MetaMorph.
I would like to make a suggestion related to your worries about control
of the laser power. Probably, you have already found yourself that you
can use your MetaMorph for neutral density filters installed into filter
wheels.

Ed

Steve Bagley wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Gabor,
>
> I have been chatting to Universal Imaging regarding exactly the same
> issue. Being able to set up complex time lapse experiments/Macros,
> such as capturing brightfield images and then a confocal image every
> so often would be extremely beneficial. Sadly the Ultraview software
> seems a little lacking in this respect.
>
> I gather than MetaMorph can control camera, exposure/gain/capture and
> the scan head but it would be difficult to control the laser power
> from MetaMorph. If anyone have heard differently please let me know as
> I would also benefit from a MetaMorph/Ultraview combination.
>
> All the best,
>
> Steve
>
>
>
>
> Steve Bagley
>
> Associate Scientist
>
> Applied Imaging Facility
>
> Paterson Institute For Cancer Research
>
> Cancer Research UK
>
> Christie Hospital, Wilmslow Rd,
>
> Manchester. M20 9BX, UK
>
>
>
> -----Original Message-----
> From: Gabor Csucs [mailto:[log in to unmask]]
> Sent: 03 May 2004 11:50
> Subject: Perkin Elmer Spinning disk under Metamorph
>
> Search the CONFOCAL archive at
>
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear All
>
> For some reasons we would like (eventually) to run our Perkin Elmer
>
> (UltraView) spinning disk system under Metamorph. I know that in
>
> principle it is possible to control a spinning disk system with
>
> Metramorph (e.g. Visitech) but is there anybody  who has experience with
>
> a Perkin-Elmer/Metamorph  combination? I'd very much appreciate your
>
> comments.
>
> Thanks      Gabor
>
> --
>
> Gabor Csucs PhD
>
> Light Microscopy Centre ETH Zürich
>
> Inst. of Biochemistry
>
> ETH Hönggerberg HPM D 8.1
>
> Tel:41-1-633-6221
>
> e-mail: [log in to unmask]
>
> ------------------------------------------------------------------------
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--
Edward Monosov, Ph.D.
Director , Cell Imaging & Histology
The Burnham Institute
10901 N. Torrey Pines Rd,
La Jolla, CA 92037
Ph:    (858)646-3100 ext. 3206
Fax: (858)646-3196
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