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Hi,
My experience has been similar to Carl's, I have almost never gotten an
actual emission spectra for a solid state light source. Hence I always
measured it myself. The manufacturer just quotes a centre wavelength and
sometimes a bandwidth. Maybe I'm overly cautious but I do the same for
optical filters too, especially older coloured glass ones. IMO it is
very important to know the actual spectrum of the light source.
Regards,
Justin.
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Boswell, Carl A - (cboswell)
Sent: Saturday, 25 February 2012 3:12 AM
To: [log in to unmask]
Subject: Re: LEDs
Hi Guy,
My reference to lack of spectra came from the online documentation for
API's InsightSSI
http://www.api.com/downloads/pdfs/lifescience/InsightSSI.pdf (no
spectra) and X-Cite's XLED1's
http://www.ldgi-xcite.com/downloads/brochures/LDGI-XCite-XLED1-FAQ-2012.
pdf separate LED spectra. Lumencor's SOLA light engine brochure
http://www.lumencor.com/products/sola_product_sheet.html is the closest
I've seen to a useable spectrum.
In fact, thanks to Lumencor's openness the SOLA spectrum is on the
database, www.spectra.arizona.edu. With the ability to juxtapose (and
download) this spectrum with spectra from the listed filters, mirrors
and labels, the value to the imaging community of making these light
source spectra available becomes more obvious.
Cheers,
C
Carl A. Boswell
520-742-6131
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Guy Cox
Sent: Friday, February 24, 2012 6:36 AM
To: [log in to unmask]
Subject: Re: LEDs
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Well, I think they do. The second edition of my book 'Optical Imaging
Techniques in Cell Biology' has spectra from one of the major players in
the field. I do think that this data is freely available. Has anyone
actually had trouble getting these spectra?
Guy
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Boswell, Carl A - (cboswell)
Sent: Friday, 24 February 2012 1:49 PM
To: [log in to unmask]
Subject: Re: LEDs
"If you have a spectrometer available I suggest scanning the emission to
get the real emission spectrum of the LED."
It would be of more general use if the manufacturer supplied a
standardized, trustworthy spectral output of these devices. Then
everyone could determine for themselves what would work best for their
systems and samples without the need for additional instrumentation.
C
Carl A. Boswell
520-742-6131
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Justin Ross
Sent: Thursday, February 23, 2012 6:14 PM
To: [log in to unmask]
Subject: Re: LEDs
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Hi,
We previously had the Xe lamps and changed to the SSI illumination on
our Deltavision Core. The exposure times were reduced by a factor of 5
to 10. The SSI is actually made by Lumencor (no commercial interest).
From my experience it works really well without the downsides of a lamp
of heat generation or having to align and change bulbs.
If you do decide to use LEDs, I strongly suggest still using bandpass
excitation filter (as suggested previously) because with some LEDs there
upto 5% light emission at wavelengths upto 50 nm higher than the peak
which will generally add a lot of background to your images. If you have
a spectrometer available I suggest scanning the emission to get the real
emission spectrum of the LED.
Regards,
Justin.
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Julio Vazquez
Sent: Friday, 24 February 2012 4:14 AM
To: [log in to unmask]
Subject: Re: LEDs
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Hello all,
Applied Precision just told me that the InsightSSI illuminator is
actually not LED based, so please ignore my previous posting, as far as
LEDs are concerned.
Julio.
==
On Feb 23, 2012, at 2:06 AM, jacques wrote:
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>
> Thanks for your help and comments !
>
> Jacques
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