CONFOCALMICROSCOPY Archives

February 2008

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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From:
Adrian Smith <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 21 Feb 2008 11:42:15 +1100
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On 19/02/2008, at 3:41 AM, Michael Weber wrote:

> Hi David,
>
> the system measures the amount of fluorescence signal per pixel and  
> controls the AOTF for this pixel based on the measured value. So if  
> there is no light coming to the detector, laser will be restricted  
> from illuminating the sample. Or, if the detector gets close to  
> saturation, laser will be restricted as well. This should reduce  
> photobleaching and phototoxicity also in out-of-focus areas. That's  
> at least how I understand this.


Does it work with the AOTF on the A1?

When I enquired about it on the C1 I was told it was only available  
with the AOM on the 3-laser board, not the AOTF on the 4 laser board?

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia

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