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Lloyd
In terms of X-ray micro tomography resolution non synchrotron systems now claim resolutions down to 50 nm (for example the Ultra from Xradia). I'm not sure how well these work in practice for wet biological material.
Ian
Ian Hallett
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Team Leader: Microscopy and Cell Walls
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Lloyd Donaldson
Sent: Wednesday, 27 October 2010 8:11 a.m.
To: [log in to unmask]
Subject: Re: water window x-ray microscopy
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Having just visited a Synchrotron where this approach was under development (they were still building the beam line and associated imaging equipment) resolution for x-ray tomography may reach sub 100 nm resolution. That is what they are aiming for but I don't think it has been achieved yet. However imaging in water may affect resolution and or contrast. Given the very intense beam it might also kill the cell since you are presumably interested in living tissue. This application is probably more suited to imaging materials.
Dr Lloyd Donaldson
Senior Scientist, Project Leader - Microscopy/Wood Identification
Scion - Next Generation Biomaterials
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Otman Benali
Sent: Wednesday, 27 October 2010 4:58 a.m.
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Subject: water window x-ray microscopy
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Hi ALL,
Water window soft X-ray microscopy is used successfully at Synchrotron
facilities to image bio samples. Water window is because water is
transparent to soft X-rays and only carbon constituents of the cell are
imaged. Could you please let me know how this techniques compares to other
microscopy techniques for bio applications.
Many thanks,
Otman BENALI, PhD
NANO-UV SAS, Paris/FRANCE
http://www.nanouv.com
http://www.mcxi.eu
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