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Hi Steffen

For any live animal work we dilute in PBS or 0.9% saline without any problems. 

Cheers

Cam 

Sent from my iPhone

On 23/07/2011, at 12:58 AM, "Steffen Dietzel" <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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> 
> Hi there,
> 
> I have used 1:5 ultrasound gel too in the past. The Ri is not identical to water but close enough for many applications. Just make sure you use water for dilution. With NaCl solution, somehow the gel structure breaks down and you get an aqueous liquid with a precipitate, at least with our ultrasound gel.
> 
> I don't know though what the 1:5 dilution in water does to exposed tissues in terms of physiology, as opposed to superfusion with buffer. Did anybody investigate?
> 
> Steffen
> 
> 
> On 22.07.2011 09:08, Cameron Nowell wrote:
>> 
>> Hi Farid,
>> 
>> For small volumes we use the Zeiss Immersol W. But for large scale stuff
>> like using the large 20x water objective on our multiphoton system we
>> use a 1:5 dilution of ultrasound gel (make sure you use the clear one,
>> not the blue tinted stuff).
>> 
> 
> 
> -- 
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
> Head of light microscopy
> 
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