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I have a couple of users with uGFP, mKO and mcherry.
No problems on our Leica SP5. The mKO is exceptionally bright in their 
samples.

Peter
CSCR, Cambridge


  On 03/11/11 16:51, Kurt Thorn wrote:
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>
> We have a couple folks who have successfully used iFP1.4 in the far 
> red (Cy5) channel on our scopes.  On our four line spinning disk 
> confocal (405/491/561/640) we have successfully done 4-color imaging 
> in mammalian cells using mTagBFP / EGFP / mCherry / iFP1.4.  Probably 
> there are better combinations out there (iRFP is supposed to be better 
> than iFP1.4) but this one works well.
>
> Kurt
>
> On 11/2/2011 3:10 PM, Cameron, Lisa wrote:
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>>
>> Hello -
>>
>> I was wondering what people's experience is with the far red FP 
>> variants - mPlum
>> or mRasberry
>> Roger Tsien's Nat. Methods paper from 2005 says mPlum is better. Have 
>> people
>> successfully been using this FP?
>> Would be great to hear recommendations/things to consider.
>> And if one would also want to image eGFP and another FP in between, 
>> what would
>> people recommend for the optimal combination of spectral separation 
>> but also
>> fluor brightness/stability?
>>
>> I have a core user who is aiming to express all three - I realize 
>> this is a
>> difficult task, just for cell health and transfection, but was hoping 
>> for FP
>> choice recommendations.
>>
>> Thank you for any feedback -
>> Feel free to contact me off list.
>> Lisa
>>
>>
>> ---------------------------------------
>> Lisa Cameron, Ph.D.
>> Director of Confocal and Light Microscopy Core
>> Dana Farber Cancer Institute
>> Boston, MA 02215
>> [log in to unmask]
>>
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