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Dear Olaf,

It is hard to give an precise answer without knowing more
details like:

1) Were the stripes present it an X-Y image or an X-T?
2) How thin are the "stripes"? One pixel or more, the whole
width of the image?
3) Are the edges of the black stripes confined to the pixels
or is there a smear/intensity gradient between the rows of
pixels?

Maybe you want to send me an image for more careful inspection?

Cheers,

Franco

Olaf Selchow wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello confocal colleagues...
>
> I have a problem with our Leica SP2 system: The images show thin
> dark, horizontal stripes. Sometimes more and sometimes less. I
> couldn't figure out yet when the stripes are stronger and when not.
> Sometimes they seem to have gone completely. It looks a little like a
> phase-problem with the line scans. But I couldn't fix it by adjusting
> the phase-correction.
>
> One more hint I got is the following: The stripes were seen the first
> time after the 2Photon system (IR laser Coherent Mira 900, EOM and
> all the extra bits and pieces) has been added to the standard SP2.
> Unfortunately this has been before I joined the lab. So I can not
> confirm this myself. Before that the images were fine (that's what
> the older colleagues here in the lab say).
>
> Independent from the 2P system I thought that the stripes could come
> from some noise on the electricity supply. We're a big institute and
> I don't know what other equipment is used in the building... and as I
> said I couldn't correlate it with any external parameter like
> daytime, weekday or something else.
>
> The images are sufficiently good quality to interpret. But they are
> no good for publication....
>
> Has anybody had such a problem before and/or have an idea about how
> to solve it?
> I'd like to avaid to call (and pay) the Leica service when it's maybe
> just a very easy thing that I can do myself.
>
> Thanks in advance for your help!
>
> Olaf
>
> --
>
>  --------------------------------------------
> | Dr. Olaf Selchow                           |
> | SFB 495 Z1 - Central Microscopy Facility   |
> | Institute for Cell Biology und Immunology  |
> | University of Stuttgart                    |
> | Allmandring 31, 70569 Stuttgart, Germany   |
> | T: +49 711 685 8209, Fax: +49 711 685 7484 |
> | email: [log in to unmask]    |
>  --------------------------------------------
>

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