Hank

We see this type of effect sometimes just prior to a laser failure [Kr/Ar].
Do you find that the orientation or spacing changes when you increase or
decrease the PMT voltage ?

Regards
Lloyd Donaldson - Microscopist
Future Forests
Forest Research, Rotorua, New Zealand
Ph 64 7 343 5581
email [log in to unmask]
www.forestresearch.co.nz/mnz



                    hpadams
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                    Microscopy List                  Subject:     moire effects
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                    25/10/01 09:01 AM
                    Please respond to Confocal
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Confocal listservers,
I have a user who is using a 10x objective to image nerve tracks in brain
tissue. He is using an aqueous mounting media which completely dries. On
some
preps he gets what looks like moire lines across his image. I have seen
this
before and thought it maybe caused by drying artifacts between coverslip
and
sections (which are picked up on the slide not the coverslip). Does anyone
have any suggestions which precludes the formation of these moire? lines
and
an explanation of their formation. Also, they seem to be wavelenght
dependent:
more pronounced with the 543 line than the 488 line. Is there a relation
with
excitation or emission?

Hank Adams
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx

Hank Adams
Manager
Integrated Microscopy Core
Molecular and Cellular Biology
Baylor College of Medicine
Houston, Tx 77030