Hi Susan,
Probably, your globular structures are the expanded ER cisterns filled
with a GFP. You can try antiPDI (Protein Disulfide Isomerase) antibody
from the "Affinity BioReagents, Inc". Phone: 800-527-4535 or
303-278-4535;  FAX: 303-278-2424;

"Garfield, Susan (NCI)" wrote:
>
> Hello everyone,
>
> When transfecting a GFP fusion protein into NIH 3T3 cells (mouse
> fibroblasts), we are seeing distribution into large globular stuctures in
> the cell cytoplasm.  We have tried staining the lysosomes, but there is no
> colocalization.  Does anyone have any suggestions as to what these could be
> and of appropriate stains for colocalization?
>
>  <<Vadim SelK localization.doc>>
>
> Thanks,
>
> Susan Garfield
> Confocal Facility Manager
> Laboratory of Experimental Carcinogenesis
> Division of Basic Sciences
> National Cancer Institute, NIH
> Building 37, Room 3C28
> 37 Convent Drive MSC4255
> Bethesda, MD  20892-4255
> +1 (301)496-5688 x227 (voice); +1 (301)496-0734 (fax)
>
>   ------------------------------------------------------------------------
>                                   Name: Vadim SelK localization.doc
>    Vadim SelK localization.doc    Type: Microsoft Word Document (application/msword)
>                               Encoding: base64

--
Edward Monosov, Ph.D.

  Director
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