Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
For deconvolving try for free www.powermicroscope.com
ALby
p.s. in september
On 19 ago 2004, at 06:41, Christian Soeller wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Guy Cox wrote:
>
>> Some points - if you want to measure a true PSF of a high NA lens
>> 100nm beads are the maximum you should consider, and 60nm which are
>> now easily available would be better. Oversample as much as you
>
> I'd dare say 100nm is plenty small enough. Since the PSF will only be
> enlarged due to the *convolution* with a 100nm bead the error with a
> 200nm in-plane resolution is ~10%. Given a 500nm out-of-plane
> resolution
> the error is down to ~2%. An if you are really fanatic about recovering
> the actual values you can deconvolve the bead (100nm sphere) from the
> recorded PSF.
>
>
> Christian Soeller
>
> --
> Christian Soeller PhD Dept. of Physiology
> University of Auckland Auckland, New Zealand
>
>
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