Hello Guy et al

We've just got a Hamamatsu ORCA C4742-95 for high resolution
fluorescence imaging of muliple labelled fixed tissues. It is based on a
Sony interline transfer chip 1280x1024 and is digitised at 12bit with
cooling to about 5C. it come with a stadard C-mount, although other
options are available. We control it using simple and easy to use
plugins for IPLab Spectrum (Scanalytics). The image quality is
outstanding - it has allowed us to get excellent images of
triple-immunolabelled preparations using a standard 4x objective.
Although the chip has maximum sensitivity for green wavelengths (an
advantage for us since we use very narrow band filters to prevent bleed
through from things like DiI and Cy3), it works really well both for
blue and far red (Cy5) fluorescence. I don't know what the lux
sensitivity is, but we can easily image everything you'd ever want to
see in completely unlablled tissue!

Of course all this is not cheap (about $A30k) - but the quality and ease
of use is all there...

hope that helps

IAN


Guy Cook wrote:
>
> Charles, what is the lux detectability level?  I am looking for a digital
> camera as well, but am primarily interested in fluorescence microscopy.
> What is your experience with the DP-10 in this area? To date, I have only
> used the Optronics cooled CCD cameras.  Any other comments from the
> listserve are appreciated.
>
> Thank you,
>
> -Guy
>
> Guy Cook
> President
> Bacterin
> 910 Technology Blvd.
> P.O. Box 6743
> Bozeman, MT 59715
> 406-582-8184
> Fax 406-586-0396
> http://www.bacterin.com
> ftp.bacterin.com
>
> > -----Original Message-----
> > From: Confocal Microscopy List
> > [mailto:[log in to unmask]]On Behalf Of Charles
> > Fanghella
> > Sent: Thursday, February 24, 2000 5:21 AM
> > To: [log in to unmask]
> > Subject: Re: camera
> >
> >
> > Katharina Braun;
> >
> > In response to your request for a digital camera to utilized to
> > capture brain tissue sections consider the following:
> > The Olympus DP-10 Digital Camera has 2.5 MegaPixels, LCD veiewing
> > screen for "Live" focus, NTSC output, and images may be saved on
> > either SmartMedia or Compact Flash cards. The main feature is
> > that the camera body has a standard "C" Mount so that it can me
> > assembled onto any microscope with a "C" Mount adapter.
> > Hope this information is helpful.
> >
> >
> > Charles Fanghella
> > Olympus America Inc
> > 1-800-645-8100 EXT 6495
> > [log in to unmask]
> >
> > >>> Katharina Braun <[log in to unmask]> 02/21/00
> > 06:12AM >>>
> > Dear cofocalists,
> > I am looking for a digital camera which can be used to take pictures of
> > stained brain tissue sections under the microscope. Recently someone
> > from this list recommended the Nikon Coolpix 950, but it is not yet
> > available for demos here in Germany so I cannot yet test it. One problem
> > which I encountered when testing the previous 800 model was that it was
> > not possible to get the microscopic images in focus, which was mainly
> > due to the camera´s small monitor, which is not large enough to focus on
> > microscopic specimens. Also the autofocus did not give real good quality
> > images. So the question is, is it the same problem with the 950 model
> > and if yes, how can I solve this. Or is there another, not too expensive
> > camera which is better equipped for our purposes?
> > Thanks for your advice,
> > Katharina Braun
> >

--
Professor Ian Gibbins
Anatomy & Histology
Flinders University of South Australia
GPO Box 2100, Adelaide, SA 5001
Australia

Phone:  +61-8-8204 5271
FAX:    +61-8-8277 0085
Email:  [log in to unmask]