 
| Subject: | |
| From: | |
| Reply To: | |
| Date: | Thu, 19 Jun 2014 23:45:52 +0000 |
| Content-Type: | text/plain |
| Parts/Attachments: |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Thanks Kate! Iıve found it very difficult to excite mCherry with the
MaiTai at the higher wavelengths, but actually down at 780nm, it excites
so well, that it photobleaches extremely quickly. But yes, I think for
more stable excitation, you really need to OPO or InSight for mCherry.
Havenıt used Cy5 much so canıt comment on it. But this is exactly why Iım
more inclined to get the OPO or InSight instead of just another Ti-Saph.
So many more options!
Dr Pamela A. Young
| Light and Optical Microscopist
Australian Centre for Microscopy & Microanalysis
THE UNIVERSITY OF SYDNEY
Rm 116A, Madsen Building F09 | The University of Sydney | NSW | 2006 |
Australia
T +61 2 9351 7527 | F +61 2 9351 7682
E [log in to unmask] | W http://sydney.edu.au/acmm
Incorporating:
Australian Microscopy & Microanalysis Research Facility (AMMRF) | W
http://www.ammrf.org.au <http://www.ammrf.org.au/>
ARC Centre of Excellence for Design in Light Metals | W
http://www.arclightmetals.org.au <http://www.arclightmetals.org.au/>
CRICOS 00026A
This email plus any attachments to it are confidential. Any unauthorised
use is strictly prohibited. If you receive this email in error, please
delete it and any attachments.
On 19/06/2014 11:25 pm, "Kate Luby-Phelps"
<[log in to unmask]> wrote:
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>In our core facility at UT Southwestern, we have three Coherent lasers: a
>refurbished Ultra
>that was a replacement for our Chameleon XR, an Ultra II and a Vision. We
>carry service
>contracts on all of them. We have found them to be very reliable and have
>experienced no
>more than a day or two of down time since 2005, and none since we got rid
>of the XR. Our
>head hours are in the thousands. The built in compensation on the Vision
>does give some
>improvement in deep penetration but there is a time lag in the adjustment
>(through Zeiss
>Zen software) that makes difficult to tweak it precisely. Don't know
>whether it is the laser or
>the software that dictates this. In 10 years we have only had to ship a
>laser back twice and
>both times Coherent sent us a replacement first. If we had to pay for
>shipping to Scotland I
>am not aware of it. We have found that two photon excitation of mcherry
>is barely possible
>with these lasers and cy5 is impossible, so if your users want to have a
>cy5 two photon
>channel, an OPO will be necessary. Not sure whether the DeepSee can do
>far red? A Watt at
>1300 nm sounds like it might do the job. Although I have no personal
>experience with SP,
>what I hear via the grapevine suggests that the coherent and sp lasers
>are equally good
>these days. My two cents.
|
|
|
