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Date: | Thu, 17 Oct 2013 20:55:36 -0500 |
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*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Hi Vitaly,
You brought up Cy3B - what is the GE web page with the quantum yield of
Cy3B, please?
If you think Molecular Probes current web site and support are bad -- in
spite of Jason's reply here - you should be looking forward to the
changes when Thermo Fisher buys Life Technologies/Invitrogen/Molecular
Probes.
Many extinction coefficients and quantum yields (not Alexa Fluor 488
however) are available in the PubSpectra XLSX file inside the zip
download at
http://works.bepress.com/gmcnamara/9/
The Excel file was the starting point for the U Arizona Spectra Database
http://www.spectra.arizona.edu/
which has additional spectra, especially multiphoton excitation.
another spectra viewer - and new dye that I learned from a Probes
salesperson's visit today, is at
http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html#product=S11200
George
On 10/17/2013 2:13 PM, Vitaly Boyko wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
>
> I have just called Molecular Probes, and, shamefully, they told me that a table with photo-chemical properties of all Alexa dyes does not exist. I have seen one with lifetime and QY data of most of Alexa dyes, but not all.
> Would you know if someone carefully compared relative photo-stability of Alexa dyes (A488 vs A568 vs A594), and optionally would also include Cy-3B and other cyanide dyes.
>
> Best regards,
>
> Vitaly
>
>
--
George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
http://works.bepress.com/gmcnamara/26/
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