Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Carolina Biological Supply (www.carolina.com) carries a solution they
call "protoslo" protozoa quieting solution.  That might help.



Date sent:              Wed, 19 May 2004 16:50:31 +0200
Send reply to:          Confocal Microscopy List <[log in to unmask]>
From:                   James Chalcroft <[log in to unmask]>
Subject:                Re: Immobilizing bugs
To:                     [log in to unmask]

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear Renato,
>
>
>
> I see that silica gel has been used by some workers for the purpose of
> immobilizing cells without killing them.
>
> The gel was formed by condensation at a physiological pH, from a
> mixture of sodium silicate, silica particles and glycerol.
>
>
>
> Another thought is that perhaps you can find some substance that
> sticks to the flagella so you might be able to confine your beasts to
> wobbling about their attached flagella. Have you tried poly-l-lysine
> treated slides/coverslips?
>
>
>
> Best wishes,
>
>
>
> Jim
>
>
>
> ________________________________
>
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Renato Sent: Wednesday, May 19, 2004 3:11 PM To:
> [log in to unmask] Subject: Immobilizing bugs
>
>
>
>
>
> Hello
>
> I have a collaborator that is trying to visualize GFP-transfected
> Leishmania, highly motile 10 um flagellated bugs. Does any one have
> experience with things like agarose or something of the kind to
> immobilize the parasites, yet keeping them alive ? Also, what would be
> a good fixative for GFP (as I recall from previous postings, there are
> chemicals that kill GFP fluorescence).
>
>
>
> Many thanks,
>
>
>
> Renato
>
>
>
> Dr. Renato Arruda Mortara
>
> Disciplina de Parasitologia
>
> Escola Paulista de Medicina - UNIFESP
>
> R. Botucatu 862 6o. andar
>
> 04023-062 São Paulo SP
>
> fone: 55 11 5579-8306
>
> fax:   55 11 5571-1095
>
> email: [log in to unmask]
>
> home page: www.ecb.epm.br/~renato
>
>

Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [log in to unmask]