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Hi Jacqui
We have a 63x 1.2NA water, a 63x 1.4NA oil and a 63x 1.3NA glycerol objectives. I would rank them in that order in terms of image quality - the glycerol lens is the best although the oil is close behind. The water lens is not so good but that may relate to our samples which have a high refractive index and hence water is not ideal. If you want to try them out with your samples let me know - your samples are quite different from ours so could behave quite differently.
Regards - Lloyd
Dr Lloyd Donaldson
Project Leader - Microscopy & Wood Identification
Manufacturing and Biomaterials
Scion - Forests, Products, Innovation
49 Sala Street, Rotorua 3010
Ph 07 343 5581
www.scionresearch.com
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Jacqui Ross
Sent: Thursday, 28 June 2012 6:47 p.m.
To: [log in to unmask]
Subject: Silicone oil immersion lenses for live cell imaging
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Dear All,
I'm currently trialling 2 silicone oil immersion lenses from Olympus (30x/1.05NA and 60x/1.3NA) for live cell imaging on our Olympus FV1000 confocal, which is on an upright fixed stage microscope. So far, they both seem excellent and offer the higher level of resolution I'm looking for.
I am particularly interested in how these lenses compare with high NA water-immersion objective lenses. At the moment, we have long working distance water-immersion lenses or standard oil immersion so I don't have a high NA water-immersion objective lens to do my own comparison.
If anyone has personal experience with both types of lenses, I would be keen to hear their view.
I'm particularly interested in how the 60x/1.2NA water-immersion lens compares to the silicone oil immersion 60x/1.3NA, since I would like to buy one or the other.
Kind regards,
Jacqui
Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND
Tel: 64 9 373 7599 Ext 87438
Fax: 64 9 373 7484
http://www.fmhs.auckland.ac.nz/sms/biru/
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