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Date: | Sat, 30 May 2009 16:51:23 -0400 |
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No experience with Lysotracker Red, but I normally run our 559 at 10%,
4ns dwell time on our FV-1000 spectral--either Cy3 or AlexaFluor 568.
Julian
Jean-Pierre CLAMME wrote:
>
> Hello,
>
> I was wondering if some of you had experience with the LysoTracker Red
> DND-99 and maybe even on the Fluoview 1000.
> I tried it out today and I was surprised by the weakness if the
> signal. I had to use over 3% of my 559 Laser line (I generally use
> 0.3 to 0.5% for Cy3 labelled particles detected on the same channel).
> It would be helpful if I could get some feed backs from your
> experience with this dye.
>
> Thank you in advance,
>
> JP
>
>
> - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
> Jean-Pierre CLAMME, PhD
> Senior Scientist
> Nitto Denko Technical
> 501 Via Del Monte
> Oceanside, CA 92058
> E-mail: [log in to unmask]
> Phone: +760.435.7065
--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC 29733
803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)
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