No experience with Lysotracker Red, but I normally run our 559 at 10%, 
4ns dwell time on our FV-1000 spectral--either Cy3 or AlexaFluor 568.
Julian

Jean-Pierre CLAMME wrote:
>
> Hello,
>
> I was wondering if some of you had experience with the LysoTracker Red 
> DND-99 and maybe even on the Fluoview 1000.
> I tried it out today and I was surprised by the weakness if the 
> signal.  I had to use over 3% of my 559 Laser line (I generally use 
> 0.3 to 0.5% for Cy3 labelled particles detected on the same channel).
> It would be helpful if I could get some feed backs from your 
> experience with this dye.
>
> Thank you in advance,
>
> JP
>
>
> - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
> Jean-Pierre CLAMME, PhD
> Senior Scientist
> Nitto Denko Technical
> 501 Via Del Monte
> Oceanside, CA 92058
> E-mail: [log in to unmask]
> Phone: +760.435.7065


-- 
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC  29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)