At 23:02 25.03.2009, you wrote:
>Hi Again List,
>
>Yesterday we tried some KY and some ultrasound 
>gel (non-blue version) with great success. 
>Mangaged to image lymphatic vessels in the mouse 
>tail without any real problems (other than 
>geting the mouse where wewanted it). The KY was 
>a bit to runny and didn't stay on the sample for 
>very long. The ultrasound gel worked really 
>well, though the image was a bit fuzzy. I am 
>thinking this is probably due to bubbles in the 
>gel. For those that have used ultrasound gel 
>have you tried degassing it to get rid of small bubbles?


Cam,

glad to hear it works for you! I did it this way:
20 ml water in 50 ml plastic tube, add 5 ml 
U-Gel, Vortex rigorously, and then either let it 
stand over night or centrifuge for 5 min in the 
cell culture centrifuge. In the centrifuge, I got 
rid of all bubles (introduced during vortexing) 
in <5min. They didn't reappear afterwards.

I saw a very noticeable difference in optical 
quality comparing U-Gel itself with a 1:5 
dilution in water, which might be considered "fuzzy".

Steffen


-- 
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

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