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  Analytical & Quantitative Light Microscopy

*Directors:* Greenfield Sluder, *University of Massachusetts Medical Center,
Worcester *and David Wolf, *Sensor Technologies LLC*


*Course Date: *May 7 - 16, 2008
Online Application
Form<http://gosnold.mbl.edu/StudentApp/StudentApp.asp?CourseID=AQLM>(PDF<http://www.mbl.edu/education/admissions/applications/pdf/aqlm.pdf>)
Deadline: *January 23, 2008*
Student Course Evaluation

*2007 Lecture Schedule* *(PDF format)* Week
1<http://www.mbl.edu/education/courses/special_topics/pdf/aqlm_wk1_07.pdf>|
Week
2
<http://www.mbl.edu/education/courses/special_topics/pdf/aqlm_wk2_07.pdf>
 A comprehensive and intensive course in light microscopy for researchers in
biology, medicine, and material sciences. This course provides a systematic
and in-depth examination of the theory of image formation and application of
video and digital methods for exploring subtle interactions between light
and the specimen. This course emphasizes the quantitative issues that are
critical to the proper interpretation of images obtained with modern
wide-field and confocal microscopes. This course is limited to 30 students.

Laboratory exercises, demonstrations, and discussions include: (1)
geometrical and physical optics of microscope image formation including
Abbe's theory of the microscope and Fourier optics; (2) interaction of light
and matter; (3) phase contrast polarization and interference microscopy for
the nondestructive analysis of molecular and fine-structural organization in
living cells; (4) fluorescence microscopy, quantification of fluorescence,
and GFP; (5) principles and application of digital video imaging, recording,
analysis, and display; (6) digital image processing and quantitative digital
image deconvolution; (7) ratiometric measurement of intracellular ion
concentrations; (8) confocal microscopy; and (9) new advances in light
microscopy such as FRET, FLIM, TIRF, and pattern illumination.

The program is designed primarily for: (1) university faculty, professional
researchers, postdoctoral fellows, and advanced graduate students in the
life sciences who wish to expand their experience in microscopy and to
understand the quantitative issues associated with analysis of data obtained
with optical microscopes; (2) individuals well-grounded in the physical
sciences, who wish to exploit microscopy techniques for analyzing dynamic
fine-structural and chemical changes; and (3) industrial scientists and
engineers interested in advancing the design of equipment and techniques
involving video and digital microscopy.

Lectures are followed by small group laboratory sessions and demonstrations.
As a result, students will have opportunities for extensive hands-on
experience with state-of-the-art optical, electronic, and digital imaging
equipment guided by an experienced staff from universities and industry.
*
**2007 Course Faculty & Lecturers:*

Edward (Ted) Salmon, *University of North Carolina, Chapel Hill*

Richard Cardullo, *University of California, Riverside*

Rainer Heintzmann, *Kings College London*

Edward Hinchcliffe, *University of Notre Dame*

John Murray, *University of Pennsylvania*

Mary-Ann Mycek,* University of Michigan*

Champika Samarasekera, *Sensor Technologies*

Randi Silver, *Weill Cornell Medical College*

Aaron Straight, *Stanford University*

Jason Swedlow, *University of Dundee*

Jennifer Waters, *Harvard Medical School*